Regenerative Repair and Medicine

Novel Stem Cell Techniques

Novel Stem Cell Techniques:
Innovative applications of cell therapy products (CTPs) derived from the Human Pluripotent Stem Cells (HPSCs) in regenerative medicine are currently being developed. The presence of residual undifferentiated HPSCs in CTPs may be a quality concern related to tumorigenicity. Here, we show a completely particular approach for the direct and sensitive detection of a trace amount of the undifferentiated Human Induced Pluripotent Stem Cells (HiPSCs) having a highly efficient amplification. Essential 8 medium has a better facilitated the expansion of HiPSCs detach into a single cells on laminin-521 than in mTeSR1 medium. Essential 8 medium allowed robust HiPSC proliferation plated on the laminin-521 at low cell density, whereas mTeSR1 didn't enhance the cell growth. HiPSCs cultured on the laminin-521 in Essential 8 medium were assisted in an undifferentiated state and they maintained the ability to differentiate into various cell types.